The specificity of anti-GPX1 was confirmed in this study by demonstrating positive staining in human liver, lung and breast carcinoma, omission of anti-GPX1 in the immunohistochemical procedure and the successful use of GPX1 antibody from the same supplier by others (Basnet et al. 2019, Chu et al. 1992; Miki et al. 2018). This evidence concerns the gene GPX1 and breast carcinoma.