Considering the pivotal role of TDP-43 in maintaining genomic integrity and given its mislocalization or nuclear clearance impairs DNA DSB repair in ALS-affected MNs [11], we examined the level of DSB marker γH2ax in cortical tissues of 12-month-old MN-Tdp-43∆NLS and sham mice by IB, which showed about fourfold higher expression of γH2ax in MN-Tdp-43∆NLS mice cortical brain samples than in sham controls (Fig. 5A-B). This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.