Possibly,the subpopulations of TNC+/CD63+ EVs may primarilyoriginate from tumor-infiltrating immune cells,45,46 since CD63 has been shown to be upregulated in glioma-associatedmacrophages,13,47 and its mRNA was herein observedto colocalize with tumor-infiltrating myeloid cells. Here, CD63 is linked to glioma.