Spatial transcriptomics analysis confirmed that TNCmRNA overexpressionspecifically colocalizes with regions of high malignant cell content,particularly with areas of glioblastoma cells with MES-like signatures.Also, TNC expression mainly occurs in tumor areas that contain relevantand frequent chromosomal aberrations in glioma, such as gain of chromosome7.9,54 This suggests that TNC is overexpressed in the genuinetumor cells, which is in agreement with our immunostaining resultsin glioblastoma tissue and previous immunohistochemical studies.55 This evidence concerns the gene TNC and glioma.