As shown in Figure 4J, Western Blotting (WB) analysis revealed that the phosphorylated protein levels of EGFR, ERK1/2, AKT, and CDK1/2 were significantly increased by Plac1 overexpression and inhibited by Plac1 knockout in HNSCC cells. This evidence concerns the gene MAPK3 and head and neck squamous cell carcinoma.