Importantly, CDH2 and ITGB1, which are implicated in influencing tumor progression, were found to be enriched in the CAF cluster, which is consistent with previous studies.[28] Taken together, these findings validated the successful capture and sequencing of all cell types from the 2D and the 3D TMEC model, accurately reflecting their respective culture conditions. The gene discussed is ITGB1; the disease is neoplasm.