To confirm the potential oncogenic function of CARHSP1 in PCa progression, we designed two short hairpin RNA (shCAR-1 and shCAR-2) to knock down the endogenous expression of CARHSP1 in PCa cells and the efficiency of knockdown was validated by western blotting analysis (Fig. 2A; Fig. S1C). This evidence concerns the gene CARHSP1 and posterior cortical atrophy.