Then, we further examined CARHSP1 expression in a panel of prostate cancer cell lines (C4-2, 22Rv1, PC-3, DU145, and LNCaP cells) and the normal prostate epithelial cell lines (RWPE-1, P69) by the western blotting analysis, and found that CARHSP1 expression was markedly increased in all five tested prostate cancer cell lines compared with two non-malignant prostate cell lines (Fig. 1E; Fig. S1A). Here, ICA1 is linked to prostate cancer.