In vitro experiments confirmed that T3 activated both LKB1/AMPK and PI3K/Akt signaling in HCC cells, while THRSP-OE activated LKB1/AMPK signaling but inhibited PI3K/Akt signaling, and THRSP-KD inhibited LKB1/AMPK signaling but activated PI3K/Akt signaling (Fig. 2G, H; Supplementary Fig. S2E, F). Here, AKT1 is linked to hepatocellular carcinoma.