In addition, a number of compounds essential for the generation of organoids, such as A83-01, SB202190, Noggin, Wnt3a, and R-spondin, were removed from the sarcoma PTC medium, since they had negligible effects on sarcoma PTC growth, whereas materials with hydrophobicity were implemented to modify the culture plate or chip in order to enhance the formation of sarcoma clusters (Figure S1A). This evidence concerns the gene WNT3A and sarcoma.