TGFB1 and wet macular degeneration: As expected, the presence of Tregs increased the concentration of TGF-β1 and IL-10 in the medium of the co-culture system, and the levels of these cytokines increased to the highest levels in the HCEC+wet AMD Treg group (Figure 4A, P<0.05, P<0.001, and P<0.0001).