Consistent with expression analysis, quantitative immunofluorescence microscopy of 19 human HCC tissue sections revealed a significant increase of plectin fluorescence intensities in tumor (T) compared to adjacent non-tumor (NT) tissue, with plectin perimembranous enrichment in tumor hepatocytes (Figure 1C; Figure 1—figure supplement 1G). This evidence concerns the gene PLEC and hepatocellular carcinoma.