FAT1 and atherosclerosis: In the present study, to investigate the impact of Fat-1 on lipid profiles and the efficacy and the safety of gene therapy for MAFLD and atherosclerosis, we first constructed an AAV9 vector expressing Fat-1 or null and then injected it via the jugular vein into WT hamsters at a dose of 1 × 1013 vg/kg, and then the animals were fed a standard chow diet (CD) for 28 weeks or an HFD containing 20% fat and 0.5% cholesterol for 24 weeks following 4-week CD (Fig. 1A).