TJP1 and early-onset autosomal dominant Alzheimer disease: Immunofluorescence microscopy is then used to evaluate tight-junction proteins (occludin, claudin-5 and zona-occluden-1 (ZO-1)), AQP4 water channels and activated microglia to understand which BBB immune cell changes may be involved with peripheral and neuroinflammatory mechanisms in Alzheimer’s disease pathogenesis.