To assess the apoptotic effects of luteolin on human DLBCL cell lines U2932 and OCI-LY10, we employed Annexin V-FITC/PI double staining and observed a significant dose-dependent increase in apoptotic cell numbers following a 24-h treatment with luteolin at concentrations of 5, 10, and 20 μM, as compared to the untreated control group. This evidence concerns the gene ANXA5 and diffuse large B-cell lymphoma.