Next, considering that we were unable to retrieve primary tumor and normal tissue of P3, we sought to understand whether our enrichment approach could be informative in determining the cancer specific origin of the c.376-1G>A TP53 mutation found in this patient’s ascites, as it could allow to annotate with certainty whether the P3 specimen was positive or negative for the presence of cancer cells despite inconclusive PAX8 staining. Here, TP53 is linked to cancer.