Compared with the blank plasmid group, after HONE1-ACSL4-WT cells were treated with RSL3, a lot of mitochondria shrank and the number of cristae decreased significantly, while the K383R of ACSL4 could weaken this effect (Fig. 9C), indicating that deacetylated ACSL4 could inhibit ferroptosis in NPC cells. The gene discussed is ACSL4; the disease is nasopharyngeal carcinoma.