To recapitulate the macrophage-conditioned 3D characteristics of the TME, we established a 3D tumor spheroid-in-Matrigel culture system using the murine pancreatic (Panc02) and colon (MC38) adenocarcinoma cell lines and treated macrophage conditioned media obtained from M0 (untreated) or M1 (IFNγ plus lipopolysaccharide-treated) macrophages (Fig. 1A, Supplementary Fig. 1A, B). This evidence concerns the gene IFNG and adenocarcinoma.