TNF and neoplasm: To see whether similar results are found in M1 macrophages polarized by stimuli other than IFNγ + LPS and M2 macrophages by IL-4 or TGF-β1, we stimulated the macrophages with a tumor conditioned media of 2D- (2D TCM) or 3D-cultured tumor cells (3D TCM), IFNγ alone, TNFα, IL-4 or TGF-β1.