BXPC-3 cells expressing doxycycline-inducible shAP2M1 or shCTRL and RFP to identify cells expressing the shRNA were cultured as tumor spheroids in the presence of doxycycline and ITGB6 at the plasma membrane detected by immunofluorescent staining with an anti-ITGB6/ITGAV antibody without permeabilizing the spheroids cell membrane. The gene discussed is ITGB6; the disease is neoplasm.