Furthermore, NEO MDM differentiation resulted in higher proportions of CD68 macrophage marker positive MDMs (Fig. 5h, Supplementary Fig. 5a, b) with increased expression of CD80 (Fig. 5i, Supplementary Fig. 5d) compared to AD MDM, while the ratio of M1-like CCR7+ and M2-like CCR7- subsets23 was similar (Fig. 5j, Supplementary Fig. 5a–c). Here, CD68 is linked to Alzheimer disease.