ITGAL and acute kidney injury: Supplemental Figure 3 provides a detailed description of our analysis using our previously published dataset (6), together with additional newly generated lung scRNA-Seq data. Despite endothelial cell upregulation of ICAM1, blockade of CD18 (Figure 4B) or LFA-1 (Supplemental Figure 4) did not reduce early neutrophil recruitment into the lungs 2 hours after AKI, suggesting that neutrophil accumulation during remote lung inflammation does not follow the classical neutrophil recruitment paradigm delineated largely in models of direct tissue injury (22).