Figures 3A-B indicate that MDA-MB-231 cells exhibited higher levels of NRP1 protein compared to the other cell lines studied, and high level of GLUT1 expression was observed in HCT116 cells, whereas NRP1 and GLUT1 were minimally expressed in NCI-H1299 cancer cells, this suggests that the cancer cell lines MDA-MB-231, HCT116 and NCI-H1299 can be utilized to assess the binding specificity of NGF for NRP1 and GLUT1. This evidence concerns the gene SLC2A1 and cancer.