Consistent with the role of Msi2 in stem cell maintenance, the inhibition of Msi2 in lung cancer cells had a significant impact on developmental and stem cell signaling programs and genes, this included Porcn, the transcription regulator Nupr1, and the NuRD complex member Mbd3, which have been shown to play a role in the maintenance of the stem cell state (Figure 5B, C; Clevers et al., 2014; Tammela et al., 2017; Zhou et al., 2018; Loughran et al., 2017). This evidence concerns the gene MBD3 and lung cancer.