To study the ameliorating effect and potential pathways of ATM on metabolic disorders in T2DM, mice livers of the NC, MD and ATM-H groups were assigned to metabolomics analysis, and the obtained data were evaluated using Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) algorithms. This evidence concerns the gene ATM and type 2 diabetes mellitus.