These two subtypes exhibited significant molecular characteristic differences across all data types (Figure 2A), including mRNA expression level differences in cell cycle regulatory genes (AURKA, AURKB, BUB1, and CDK1), expression profile differences in long non-coding RNAs (LINC00261 and SFTA1P), expression differences in microRNAs (particularly hsa-mir-31 and hsa-mir-196b), differences in DNA methylation patterns, and mutation frequency differences in cancer-associated genes (especially TP53 and MUC16). Here, BUB1 is linked to cancer.