The dual luciferase reporter gene assay confirmed that ZNF32 H179A and H183A transcriptionally activate ISY1-RAB43 and UPK3BL1 as well as inhibit the transcription of SNX22. Our study thus deepens the understanding of the functions of ZNF32 mutants as well as NSs in breast cancer cells while providing a basis for finding new treatments for breast cancer. Here, UPK3BL1 is linked to breast carcinoma.