In this study, we validated that ZNF32 H179A, H183A, and H179, 183A promote NS formation; however, in vitro and in vivo experiments suggest that only ZNF32 H179A and H183A promote the proliferation of breast cancer cells through the loss of one imidazole ring on the fourth zinc finger structure as well as differential upregulation of ISY1-RAB43 and UPK3BL1 along with downregulation of SNX22 expressions (Figure 8). This evidence concerns the gene ISY1 and breast cancer.