Additionally, we also investigated the prognostic value of UBA1/6 in pan-cancer from GEO database, and discovered that elevated UBA1 expression in breast cancer (GSE1456), gastric cancer (GSE14210), and myeloma (GSE24080) is associated with a poorer prognosis, whereas higher UBA1 expression correlates with a more favorable prognosis in colon cancer (GSE1258)., Notably, the relationship between UBA6 expression and prognosis did not reach statistical significance in the aforementioned dataset. Here, UBA6 is linked to breast cancer.