Additionally, we also investigated the prognostic value of UBA1/6 in pan-cancer from GEO database, and discovered that elevated UBA1 expression in breast cancer (GSE1456), gastric cancer (GSE14210), and myeloma (GSE24080) is associated with a poorer prognosis, whereas higher UBA1 expression correlates with a more favorable prognosis in colon cancer (GSE1258)., Notably, the relationship between UBA6 expression and prognosis did not reach statistical significance in the aforementioned dataset. The gene discussed is UBA6; the disease is malignant colon neoplasm.