Next, we performed TUNEL staining of the tumor tissues of mice in each group, which showed that green fluorescence was notably increased in the 5-FU group and the 5-FU + M0-exos group compared with the control group, but decreased in the 5-FU + GRP78-exos group, suggesting that the treatment of GRP78-exos caused a decrease in TUNEL-positive cells and suppressed the killing effect of 5-FU on CRC cells (Fig. 3F, G). This evidence concerns the gene HSPA5 and colorectal carcinoma.