We generated cancer-relevant R2587C, L2624F and D2629H mutants of ASH1LPHD and found that binding of these mutants, especially of R2587C, to H3K4me3 peptide was diminished, suggesting that the impaired function of ASH1LPHD could be potentially linked to aberrant activity of ASH1L (Supplementary Fig. 14). This evidence concerns the gene ASH1L and cancer.