To further elucidate the significance of ERRα in ZIP1 regulation and its contribution to the growth of PCSCs, we performed the shRNA-mediated knockdown of ZIP1 in ERRα-knockdowned prostate cancer cells (Fig. 8b), and found that ZIP1 knockdown could abrogate the intracellular zinc accumulation (Fig. 8c, d), and restore the suppressed OXPHOS status (Fig. 8e), ATP production (Fig. 8f), in vitro spheroid formation capacity (Fig. 8g, h) as well as in vivo tumorigenicity (Fig. 8i; Supplementary Fig. S5c) induced by ERRα knockdown in prostate cancer cells. Here, ESRRA is linked to Familial prostate cancer.