Considering these differences, it is unsurprising that up to a third of spatial epidemiological studies on leptospirosis described in the literature rely on detection through ELISA testing [32], following the World Health Organization (WHO) endorsement of IgM ELISA for the serodiagnosis of leptospirosis in areas with limited healthcare resources [33]. The gene discussed is CD40LG; the disease is leptospirosis.