CDKL5 and Anxiety: 3I-J). This suggests that the mutant mice had difficulty adapting to a new environment and showed hyperactivity even after a 30-min habituation period, indicating increased anxiety. Similarly, in the light-dark transition test, 492stop/Y mice displayed a significant preference for the dark chamber (62%), avoiding the unknown chamber and exhibiting less exploration than WT littermates (Fig. 3K). These findings are consistent with the impaired coordination and increased anxiety reported in other Cdkl5-KO mouse models [47].