qRT‐PCR and western blot results showed that GEM‐resistant pancreatic cancer cell‐derived EVs promoted a significant upregulation of α‐SMA as well as an increase in Collagen‐1 and Fibronectin compared with parental cell‐derived EVs; immunofluorescence showed the same effect (Figure 1E–G; Figure S2E–G, Supporting Information). This evidence concerns the gene ACTA1 and familial pancreatic carcinoma.