Generally, cells express Met and respond to HGF by activating PI3K/AKT and ERK1/2 signalling.[22] To evaluate the role of HGF in the protective effects of 3D‐EVs against sepsis‐induced ALI and epithelial barrier damage, we performed HGF silencing using siRNA in vitro (Figure8A). This evidence concerns the gene MAPK3 and Sepsis.