FFPE tissue sections from 10 ALK fusion-positive lung cancers, 1 EWSR1 fusion-positive sarcoma, and 35 ALK fusion-negative lung cancer cases (as confirmed by the routine fluorescence in situ hybridization [FISH] assay; Figure S1) were subjected to a 19-gene lung panel (Table S1) targeted RNA-seq assay using AMP. The gene discussed is ALK; the disease is lung cancer.