To further clarify the effect of ASF1B on the biological characteristics of GC cells, we used CRISPR-case9 gene editing technology to construct MKN45 and HGC27 gastric cancer cell ASF1B−/− knockout cell lines and constructed AGS and HGC27 GC cell ASF1B+/+ overexpression cell lines using ASF1B overexpression vectors. This evidence concerns the gene ASF1B and gastric cancer.