NCI-H157 and H460 cells, representing the main subtypes of NSCLC originating from squamous and large cell carcinoma, respectively, have been used to investigate the effects of ophiopogonin B. In these cells, ophiopogonin B inhibited phosphorylated AKT (p-AKT) at the Ser308 and Thr473 sites and significantly induced autophagy without triggering apoptosis. Here, AKT1 is linked to large cell carcinoma.