Upon measuring intestinal AMPK protein levels, we observed significant suppression in PCOS model rats, notably in the ratio of phosphorylated to total AMPK (p-AMPK/AMPK) as shown in Figure 2A (mean ± SEM: Model: 0.1692 ± 0.05284, Met: 0.5515 ± 0.09490). Here, PRKAA1 is linked to polycystic ovary syndrome.