Similar results emerged when analyses additionally controlled for any changes in leukocyte subset abundance over time (using mRNA markers of CD4+ and CD8+ T cells, B cells, NK cells, and monocytes), demographic characteristics (e.g., age, ethnicity, education, marital status, employment status, income, etc.), pre-existing conditions (depression, anxiety, PTSD, smoking, HLD, DM, HTN, mindfulness practice, etc.)or behavioral and psychometric measures that were provided. This evidence concerns the gene CD8A and major depressive disorder.