To explore YEATS2 function in ESCC, we firstly knocked down YEATS2 expression in ESCC cell lines KYSE450 and KYSE180 by transfecting siRNAs targeting YEATS2, and increased YEATS2 expression in TE5 by transfecting over-expression plasmids of YEATS2 (Supplementary Figures S1A, B). The gene discussed is YEATS2; the disease is esophageal squamous cell carcinoma.