To further verify YEATS2 function in ESCC cell lines, we established stable cell lines for YEATS2 knockdown by using CRISPR/Cas9 system in KYSE150 and KYSE180, and stable cell lines for YEATS2 overexpression by using CRISPR/dCas9-SAM system in TE5 and TE9 (Figure 2A; Supplementary Figures S1C–D). The gene discussed is YEATS2; the disease is esophageal squamous cell carcinoma.