Specifically, we identified these mutated genes in the pLN– group were associated with multiple signalling pathways involved in tumourigenesis of HNSCC (CSDM3, FAT1, and NOTCH1), and cell cycle dysregulation (CASP8, PRDM9, and SYNE1) (Figure 2C). The gene discussed is PRDM9; the disease is head and neck squamous cell carcinoma.