To elucidate whether the repression of the DREAM complex target can also occur in a p21-independent manner, we used three asynchronously growing isogenic human CRC cell lines, HCT116 p53+/+, HCT116 p53−/− and HCT116 p21−/− (Appendix Fig. S1A) which would mimic the physiological scenario in a vast majority of human tissues. The gene discussed is TP53; the disease is colorectal carcinoma.