Furthermore, 33 (66%) out of 50 normal samples, showed moderate PYGO2 expression in the ESCC tissues, 15 (30%) showed negative PYGO2 expression, and only 2 (4%) showed strong expression of PYGO2 in the ESCC tissues (Table 2, Figure 2). This evidence concerns the gene PYGO2 and esophageal squamous cell carcinoma.