Indeed, we found that TSS-A hypomethylation was closely involved in various processes of the immune system, activating multiple immune regulation pathways, such as IL2/SATA5 signaling, IFN-gamma and -alpha response, KRAS signaling, CD28 dependent PI3K/AKT signaling, T cell activation, Cytokine signaling, etc. Furthermore, the immune infiltration analyses on basis of RNA sequencing data have also revealed that AML with hypomethylation of TSS-A linked to higher immune cells infiltration, predominately with adaptive immune cells (e.g. CD4+ T cells, CD8+ T cells, and dendritic cells, etc.). This evidence concerns the gene KRAS and acute myeloid leukemia.