In line with the ESTIMATE method, the higher ImmuneScore and MicroenvironmentScore were also indicated in xCell; 2) AML with TSS-A hypomethylation was significantly infiltrated with several anti-cancer immune cells, such as CD4+ T cells, CD8+ T cells, and dendritic cells, consistently in 2-4 different algorithms. Here, CD4 is linked to acute myeloid leukemia.