Multi-ROC analysis revealed that the overexpression of MAT2B predicted the proliferative subclass of intrahepatic cholangiocarcinoma with an area under the curve (AUC) of 0.92, FH with an AUC of 0.90, PLOD1 with an AUC of 0.89, PLOD2 (two distinct quantification) with an AUC of 0.84, PDE6C with an AUC of 0.69, ALDOC with an AUC of 0.66, and NT5DC3 with an AUC of 0.62 (Fig. 4A). This evidence concerns the gene PDE6C and intrahepatic cholangiocarcinoma.