Univariate logit binomial analysis, with the outcome being the proliferative subclass status of intrahepatic cholangiocarcinoma, confirmed the significance of the probes for seven enzymes: FH, MAT2B, PLOD2, PLOD1, PDE6D, ALDOC, and NT5DC3 (Fig. 3C and Supplemental Table 2). The gene discussed is PDE6D; the disease is intrahepatic cholangiocarcinoma.