To verify data quality and preprocessing, we examined group differences for four CSF proteins that are well-known to change in abundance in AD: neurofilament light polypeptide (NEFL) [48–50], 14-3-3 protein gamma (YWHAG) [51, 52], neuronal pentraxin-2 (NPTX2) [53, 54] and fatty acid-binding protein - heart (FABP3) [55, 56]. Here, NEFL is linked to Alzheimer disease.