Similarly, an analysis of adult BAL specimens (12 PCP and 20 non-PCP cases, including 1 patient who was HIV-positive with PCP), detected PCP cases with 91.7% and 83.3% clinical sensitivity and 100.0% specificity (Figure 5C, Supplemental Figure 4B, and Table 2), while Nad4 RT-qPCR results had 66.7% diagnostic sensitivity and 94.7% specificity. The gene discussed is MT-ND4; the disease is pneumocystosis.