In vivo biodistribution studies revealedcomparable tumor uptake across all three compounds with P18 displayinghigher spleen accumulation, likely due to phenyl ring lipophilicity.These SAR findings provide a strategic framework for the rationaldesign of PSMA ligands, paving the way for the development of next-generationtheranostic agents for PCa. The gene discussed is FOLH1; the disease is neoplasm.