We used RT-PCR primers specific for CircPVT1 to demonstrate that it was significantly more abundant in cancer cell lines with MYC/PVT1 genomic rearrangements (MYC/PVT1 gain + PVT1 translocation, COLO-320DM, SK-PN-DW, and D458) compared to MYC/PVT1 neutral cell lines (U2OS, BxPC-3, and DU145) (Figure 2F). Here, MYC is linked to cancer.