In high-shedding cancer types, such as colorectal cancer (CRC, median TF 4%), small cell lung cancer (SCLC, 25.9%), and liver cancer (9.9%), bTMB≥10 was detected with an equal or greater frequency than TMB≥10 (CRC: 9% TMB≥10 versus 15% bTMB≥10; SCLC: 37% versus 33%; liver: 4% versus 9%). Here, TF is linked to cancer.