To investigate the role of MNX1-AS1 in NSCLC proliferation, we detected the expression of MNX1-AS1 in several lung cancer cell lines, and then selected A549 and H1975, which exhibited high MNX1-AS1 expression, to stably knock down MNX1-AS1 via lentivirus-mediated delivery of shRNA (Fig. 1A and B). The gene discussed is MNX1; the disease is non-small cell lung carcinoma.