The WB assay was used to determine the expression level of Protein Phosphatase 1 Catalytic Subunit Alpha (PPP1CA) following ATP1B3 deletion, immunoprecipitation was used to determine the direct influence of the two proteins, and immunofluorescence was used to analyze the distribution of ATP1B3 and PPP1CA proteins in glioma cells. The gene discussed is ATP1B3; the disease is glioma.